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Biophotonics: Optical Nanoscopy and Quantitative Phase Microscopy
June 30, 2021 @ 5:00 pm - 6:00 pm
Abstract: Optical Nanoscopy is super-resolution imaging technique by means of breaking the diffraction limit of the conventional optical microscopes. In this talk I will be discussing about the diffraction limit of microscopes and various methods for breaking the diffraction limit, such as structured illumination, stimulated emission depletion method and single molecule imaging techniques to achieve super-resolution. 2014 Nobel prize in Chemistry was awarded for Nanoscopy using these techniques. Microscopy or Nanoscopy is not only meant for the visualization of micro- and nano-organisms, but one can precisely quantify various parameters of these objects. In the second part I will be discussing about the quantitative phase microscopy using which one can measure various static and dynamic biophysical parameters. Quantitative information about these parameters leads to various disease detection at early stage. Starting with Zernike’s phase contrast, Gabor’s lens less microscopy to advanced techniques like digital holographic microscopy, interferometric microscopy and diffraction phase microscopy will be discussed. These techniques have become important tools in modern biology, such as quantification of sperm cells for IVF technology, sickle cell quantification to microphages and cancer cell analysis. Further advancement in these techniques are taking place by means of incorporating artificial intelligence, machine learning and deep learning.
Note: The webinar is free to attend, however, registration is mandatory.